La mono- y dihidroxilación selectiva de compuestos aromáticos y terpenos es una reacción altamente demandada, ya que los productos resultantes suelen ser moléculas de gran relevancia para aplicaciones industriales. Enzimas como las mono- y dioxigenasas pueden llevar a cabo estas reacciones en condiciones suaves y ecológicas. Para mejorar la actividad y selectividad de estas enzimas hacia productos de interés, empleamos ingeniería de enzimas.

Publicaciones

1. Wissner, J. L., Almeida, J. R., Grilo, I. R., Oliveira, J.F., Brízida, C., Escobedo-Hinojosa, W., Pissaridou, P., Vasquez, M. I., Cunha, I., Sobral, R. G., Vasconcelos, V. and Gaudêncio, S. P. Novel metabolite madeirone and neomarinone extracted from Streptomyces aculeoletus as marine antibiofilm and antifouling agents. Front. Chem. 12:1425953 (2024); Impact factor: 3.8
2. Wissner, J. L., Parada-Fabián, J. C., Márquez-Velázquez, N. A., Escobedo-Hinojosa, W., Gaudêncio, S. P., Prieto-Davó, A. Diversity and bioprospection of Gram-positive bacteria derived from a Mayan sinkhole. Microb. Ecol. 87, 77 (2024); Impact factor: 3.3
3. Wissner, J. L., Parada-Fabián, J. C., Márquez-Velázquez, N. A., Escobedo-Hinojosa, W., Gaudêncio, S. P., Prieto-Davó, A. Diversity and bioprospection of Gram-positive bacteria derived from a Mayan sinkhole. PREPRINT available at Research Square (2024).
4. Escobedo-Hinojosa, W., Vila, M. A., Wissner, J. L., Härterich, N., Horz, P., Iglesias, C., & Hauer, B. Exploring the substrate scope of glycerol dehydrogenase GldA from E. coli BW25113 towards cis-dihydrocatechol derivatives. J. Biotechnol. 366, 19–24 (2023); Impact factor: 4.1
5. Escobedo-Hinojosa, W., Hammer, S. C., Wissner, J. L. & Hauer, B. Fast and easy synthesis of the non-commercially available standard isobutyl monophosphate (ammonium salt). MethodsX 8, 101455 (2021); Impact factor: 1.7
6. Wissner, J. L., Schelle, J. T., Escobedo‐Hinojosa, W., Vogel, A. & Hauer, B. Semi‐Rational Engineering of Toluene Dioxygenase from Pseudomonas putida F1 towards Oxyfunctionalization of Bicyclic Aromatics. Adv. Synth. Catal. 363, Front cover picture (2021); Impact factor: 4.1
7. Wissner, J. L., Schelle, J. T., Escobedo-Hinojosa, W., Vogel, A. & Hauer, B. Semi‐Rational Engineering of Toluene Dioxygenase from Pseudomonas putida F1 towards Oxyfunctionalization of Bicyclic Aromatics. Adv. Synth. Catal. 363, 4905-4914 (2021); Impact factor: 5.4
8. Farr, T., Wissner, J. L. & Hauer, B. A simple and efficient method for lyophilization of recombinant E. coli JM109 (DE3) whole-cells harboring active Rieske non-heme iron dioxygenases. MethodsX 8, 101323 (2021); Impact factor: 1.7
9. Escobedo-Hinojosa, W., Wissner, J. L. & Hauer, B. A real-time 31P-NMR-based approach for the assessment of glycerol kinase catalyzed monophosphorylations. MethodsX 8, 101285 (2021); Impact factor: 4.1
10. Wissner, J. L., Escobedo-Hinojosa, W., Vogel, A. & Hauer, B. An engineered toluene dioxygenase for a single step biocatalytical production of (-)-(1S,2R)-cis-1,2-dihydro-1,2-naphthalenediol. J. Biotechnol. 326, 37–39 (2021); Impact factor: 4.1
11. Wissner, J. L., Ludwig, J., Escobedo-Hinojosa, W. & Hauer, B. Strategy for identification of cis-dihydrodiendiol-degrading dehydrogenases in E. coli BW25113. MethodsX 7, 101143 (2020); Impact factor: 1.7
12. Wissner, J. L., Ludwig, J., Escobedo-Hinojosa, W. & Hauer, B. An enhanced toluene dioxygenase platform for the production of cis-1,2-dihydrocatechol in Escherichia coli BW25113 lacking glycerol dehydrogenase activity. J. Biotechnol. 325, 380–388 (2020); Impact factor: 4.1
13. Wissner, J. L., Escobedo-Hinojosa, W., Heinemann, P. M., Hunold, A. & Hauer, B. Chapter Three - Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries. In Methods in Enzymology 644th ed., 63–93 (2020); Impact factor: 1.7

Circuito de Posgrado, Ciudad Universitaria
Alcaldía Coyoacán, C.P. 04510, México, CDMX

55 5623 7006
mdcbq@posgrado.unam.mx