1. Determinación de las respuestas moleculares asociadas a estrés térmico en la producción de proteínas recombinantes de M. tuberculosis en cultivos inducidos térmicamente por E. coli.
2. Producción de glicoproteínas recombinantes en S. lividans.
3. Cultivo sumergido del hongo filamentoso Humphreya coffeata para la producción de metabolitos bioactivos.
4. Producción de micelio de hongos formadores de micorriza arbuscular.
5. Desarrollo de procesos de cultivos bacterianos y su escalamiento para la producción comercial de biofertilizantes.

Publicaciones

Muñoz-Echeverri LM, Benavides-López S, Geiger O, Trujillo-Roldán MA, Valdez-Cruz NA (2024) Bacterial extracellular vesicles: biotechnological perspective for enhanced productivity. World Journal of Microbiology and Biotechnology. 40:174 https://doi.org/10.1007/s11274-024-03963-7 [2022 journal impact factor 4.1]. Q2.
Ángeles-Argáiz RE, Lozano Aguirre-Beltrán LF, Hernández-Oaxaca D, Quintero-Corrales C, Trujillo-Roldán MA, Castillo-Ramírez S, Garibay G (2024) Assembly collapsing versus heterozygosity oversizing: Detection of homokaryotic and heterokaryotic Laccaria trichodermophora strains by hybrid genome assembly. Microbial Genomics. 10(3). https://doi.org/10.1099/mgen.0.001218. [2022 journal impact factor 3.9]. Q1.
González-Hernández RA, Valdez-Cruz NA, Trujillo-Roldán MA *. Factors that influence the extraction methods of terpenes from natural sources. Chem. Pap. (2024). https://doi.org/10.1007/s11696-024-03339-z. [2022 journal impact factor 2.2]. Q3.
Valdez‑Cruz NA, Rosiles-Becerril D, Martínez-Olivares CE, García‑Hernández E, Cobos-Marín L, Garzón D, López-Salas FE, Zavala G, Luviano A, Olvera A, Alagón A, Ramírez OT, Trujillo‑Roldán MA. Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19. Microbial cell factories (2024). 23, 41. https://doi.org/10.1186/s12934-024-02320-5. ISSN 1475-2859. [2022 journal impact factor 6.4]. Q1
Sánchez-Vargas J, Valdes-Parada F, Trujillo-Roldán MA, Lasseux D. Macroscopic model for generalised Newtonian inertial two-phase flow in porous media. J. Fluid Mech. (2023) 970, A19. doi:10.1017/jfm.2023.615. [2022 journal impact factor 3.7]. Q1.
González‑Hernández RA, Valdez‑Cruz NA, Macías‑Rubalcava ML, Trujillo‑Roldán MA *. Overview of fungal terpene synthases and their regulation. World Journal of Microbiology and Biotechnology. (2023) https://doi.org/10.1007/s11274-023-03635-y. [2022 journal impact factor 4.1]. Q2.
Restrepo-Pineda S, Rosiles-Becerril D, Vargas-Castillo AB, Ávila-Barrientos LP, Luviano A, Sánchez-Puig N, García Hernández E, Pérez NO, Trujillo-Roldán MA, Valdez-Cruz NA. Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli. Electronic Journal of Biotechnology. (2022) 59: 96-106 https://doi.org/10.1016/j.ejbt.2022.08.004. [2021 journal impact factor 2.826]. Q3.
Serrano-Carreón L, Aranda-Ocampo S, Balderas-Ruíz KA, Juárez AM, Leyva E, Trujillo-Roldán MA, Valdez-Cruz NA, Galindo E. A case study of a profitable mid-tech greenhouse for the sustainable production of tomato, using a biofertilizer and a biofungicide. Electronic Journal of Biotechnology. (2022) 59: 13-24. https://doi.org/10.1016/j.ejbt.2022.06.003. [2021 journal impact factor 2.826]. Q3.
Avila-Barrientos LP, Cofas-Vargas LF, Agüero-Chapin G, Hernández-García E, Ruiz-Carmona S, Valdez-Cruz NA, Trujillo-Roldán MA, Weber J, Ruiz-Blanco YB, Barril X, Garcia-Hernandez E. Computational design of inhibitors targeting the catalytic subunit of Escherichia coli FOF1-ATP synthase. Antibiotics 2022, 11(5):557; https://doi.org/10.3390/antibiotics11050557. Online ISSN: 2079-6382. [2021 journal impact factor 5.222]. Q2.
Restrepo-Pineda S, Sánchez-Puig N, Pérez NO, García-Hernández E, Valdez-Cruz NA, Trujillo-Roldán MA*. The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli. Applied Microbiology and Biotechnology. (2022) 106(8):2883-2902. https://doi.org/10.1007/s00253-022-11908-z. ISSN: 0175-7598 (print); 1432-0614 (web) [2021 journal impact factor 5.560]. Q1.
Pérez-Rodriguez S., Reynoso-Cereceda GI, Valdez-Cruz NA, Trujillo-Roldán MA*. A comprehensive comparison of mixing and mass transfer in shake flasks and their relationship with MAb productivity of CHO cells. (2022). Bioprocess and Biosystems Engineering. https://doi.org/10.1007/s00449-022-02722-y. Electronic ISSN 1615-7605, Print ISSN 1615-7591. [2021 journal impact factor 3.434]. Q2
Posadas D, Palacios C, Zenit R, Blancas-Cabrera A, Trujillo-Roldán MA, Salinas M, Ascanio G. Flow patterns of multiple axial-radial impellers for potential use in aerated stirred tanks. Chemical Engineering & Technology. 45(5):860-867 (2022) https://doi.org/10.1002/ceat.202100521. Online ISSN:1521-4125. [2021 journal impact factor 2.215]. Q3
Campos-López A, Uribe-López JA, Cázares-Ordoñez V, Garibay-Orijel R, Valdez-Cruz NA, Trujillo-Roldán MA*. Quercetin and 1-methyl-2-oxindole mimic root signaling that promotes spore germination and mycelial growth of Gigaspora margarita. Mycorrhiza. (2022) 32, 177–191 https://doi.org/10.1007/s00572-022-01074-5. Electronic ISSN 1432-1890, Print ISSN 0940-6360. [2021 journal impact factor 3.856]. Q1
López-Taborda JD, Vargas-Zapata A, Ramírez-Vargas JF, Valdez-Cruz NA, Trujillo-Roldán MA, Orozco-Sánchez F. Development, and characterization of an alternative control system to study separately oxygen and momentum transfer effects in stirred tank bioreactors. Revista Mexicana de Ingeniería Química. (2022) 21(1):Proc2607. https://doi.org/10.24275/rmiq/Proc2607. ISSN 2395-8472. [2021 journal impact factor 2.093]. Q3
García-Cabrera RI, Valdez-Cruz NA, Daniel-Vázquez A, Blancas-Cabrera A, Trujillo-Roldán MA* Roles of culture media and oxygen transfer in the scale-up from shake flasks to pneumatic bioreactor of the plant growth-promoting bacterium Rhizobium phaseoli. Revista Mexicana de Ingeniería Química. (2021). 20(2):1093-1111. https://doi.org/10.24275/rmiq/Bio2297. ISSN 2395-8472. [2021 journal impact factor 2.093]. Q3
Restrepo-Pineda S, Pérez NO, Valdez-Cruz NA, Trujillo-Roldán MA*. Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights. (2021). FEMS Microbiology Reviews, fuab023, https://doi.org/10.1093/femsre/fuab023. Online ISSN 1574-6976. Print ISSN 0168-6445. [2021 journal impact factor 15.177]. Q1
Valdez-Cruz NA, García-Hernández E, Espitia C, Cobos-Marín L, Altamirano C, Bando-Campos CG, Cofas-Vargas LF, Coronado-Aceves EW, González-Hernández RA, Hernández-Peralta P, Juárez-López D, Ortega-Portilla PA, Restrepo-Pineda S, Zelada-Cordero P, Trujillo-Roldán MA*. Integrative overview of antibodies against SARS-CoV-2 and their possible applications in COVID-19 prophylaxis and treatment. Microbial Cell Factories. (2021). 20:88. https://doi.org/10.1186/s12934-021-01576-5. ISSN 1475-2859. [2021 journal impact factor 6.352]. Q1
Pérez-Rodriguez S, Wulff T, Gunnar Voldbor B, Altamirano C, Trujillo-Roldán MA, Valdez-Cruz NA. Compartmentalized proteomic profiling outlines the crucial role of the Classical Secretory Pathway during recombinant protein production in Chinese hamster ovary cells. ACS Omega (2021). 6(19):12439–12458. https://doi.org/10.1021/acsomega.0c06030 ISSN: 2470-1343 (print); 2470-1343 (web). [2021 journal impact factor 4.132]. Q2
Pérez-Rodriguez S, Ramírez-Lira MJ, Wulff T, Gunnar Voldbor B, Ramírez OT, Trujillo-Roldán MA, Valdez-Cruz NA. Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis. PLoS ONE (2020) 15(8): e0237930. https://doi.org/10.1371/journal.pone.0237930. ISSN: 1932-6203. / 2470-1343 [2021 journal impact factor 3.752]. Q2
Ortega-Quintana FA, Trujillo-Roldán MA, Botero-Castro H, Alvarez H. Modeling the interaction between the central carbon metabolism of Escherichia coli and bioreactor culture media. Biochemical Engineering Journal. (2020). 163:107753. https://doi.org/10.1016/j.bej.2020.107753. ISSN: 1369-703X. [2021 journal impact factor 4.446]. Q1
Pérez-Rodriguez S, Ramírez OT, Trujillo-Roldán MA, Valdez-Cruz NA. Comparison of protein precipitation methods for sample preparation prior to proteomic analysis of Chinese hamster ovary cell homogenates. Electronic Journal of Biotechnology. (2020) 48:86-94. https://doi.org/10.1016/j.ejbt.2020.09.006. ISSN: 0717-3458. [2021 journal impact factor 2.826]. Q1
García-Cabrera RI, Valdez-Cruz NA, Blancas-Cabrera A, Trujillo-Roldán MA*. Oxygen transfer affect polyhydroxybutyrate production and oxidative stress response in submerged cultures of Rhizobium phaseoli. Biochemical Engineering Journal. (2020) 162:107721. https://doi.org/10.1016/j.bej.2020.107721. ISSN: 1369-703X. [2021 journal impact factor 4.446]. Q1
Pérez-Rodríguez S, Ramírez-Lira MJ, Trujillo-Roldán MA, Valdez-Cruz NA. Nutrient supplementation stimulates culture longevity, monoclonal antibody production and oxidative metabolism of Chinese hamster ovary cells. Bioengineered (2020) 11(1):463-471. https://doi.org/10.1080/21655979.2020.1744266. Print ISSN: 2165-5979 Online ISSN: 2165-5987. [2021 journal impact factor 6.832]. Q1
Ángeles-Argáiz RE, Carmona-Reyes IA, Quintero-Corrales CA, Medina-Macías FJ, Blancas-Cabrera A, Valdez-Cruz NA, Ulloa M, Trujillo-Roldán MA, Garibay-Orijel R. From field sampling to pneumatic bioreactor mycelia production of the ectomycorrhizal mushroom Laccaria trichodermophora. Fungal Biology (2020) 124(1-2):205-218. https://doi.org/10.1016/j.funbio.2020.02.003. ISNN: 1878-6146. [2021 journal impact factor 2.910]. Q3
Arregui L, Ayala M, Gómez-Gil X, Gutiérrez-Soto G, Hernández-Luna CE, Herrera de los Santos M, Levin L, Rojo-Domínguez A, Romero-Martínez D, Saparrat MCN, Trujillo-Roldán MA, Valdez-Cruz NA. Laccases: structure, function, and potential application in water bioremediation. Microbial Cell Factories (2019) 18:200. https://doi.org/10.1186/s12934-019-1248-0. ISSN: 1475-2859. [2021 journal impact factor 6.352]. Q1
Restrepo-Pineda S, Bando-Campos CG, Valdez-Cruz NA, Trujillo-Roldán MA* (2019) Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: The role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies. Cell Stress and Chaperones. 24(4):777-792. https://doi.org/10.1007/s12192-019-01006-x. ISSN: 1355-8145 (Print) 1466-1268 (Online). [2021 journal impact factor 3.827]. Q3
Gamboa-Suasnavart RA, Marín-Palacio LD, López-Griego L, Córdova-Aguilar MS, Valdez-Cruz NA, Trujillo-Roldán MA* (2019) Volumetric power input as a reliable parameter for scale-up from shake flask to stirred-tank bioreactor: production of a recombinant glycoprotein by Streptomyces lividans. Revista Mexicana de Ingeniería Química. 18, (3) 1083-1097. DOI: doi.org/10.24275/uam/izt/dcbi/revmexingquim/2019v18n3/Gamboa. ISSN 2395-8472. [2021 journal impact factor 2.093]. Q3
Bando-Campos G, Juárez-López D, Román-González SA, Castillo-Rodal AI, Olvera C, López-Vidal Y, Arreguín-Espinosa R, Espitia C, Trujillo-Roldán MA, Valdez-Cruz NA. Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection. Microbial Cell Factories (2019) 18:11. https://doi.org/10.1186/s12934-019-1059-3. ISSN: 1475-2859. [2021 journal impact factor 6.352]. Q1
de Marco A, Ferrer-Miralles N, Garcia-Fruitós E, Mitraki A, Peternel S, Rinas U, Trujillo-Roldán MA, Valdez-Cruz NA, Vázquez E, Villaverde A. Bacterial inclusion bodies are industrially exploitable amyloids. FEMS Microbiology Reviews. (2019) 43(1) 53-72, fuy038. doi: 10.1093/femsre/fuy038. Online ISSN 1574-6976 Print ISSN 0168-6445. [2021 journal impact factor 15.177]. Q1.
Gamboa-Suasnavart RA, Valdez-Cruz NA, Gaytan-Ortega G, Reynoso-Cereceda GI, Cabrera-Santos D, López-Griego L, Klöckner W, Büchs J, Trujillo-Roldán MA* The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks. Microbial Cell Factories. (2018) 17:189. DOI: 10.1186/s12934-018-1035-3. [2021 journal impact factor 6.352]. Q1
Calcines-Cruz C, Olvera A, Castro-Acosta RM, Zavala G, Alagón A, Trujillo-Roldán MA, Valdez-Cruz NA. Recombinant-phospholipase A2 production and architecture of inclusion bodies are affected by pH in Escherichia coli. International Journal of Biological Macromolecules (2018). 108:826-836. doi:10.1016/j.ijbiomac.2017.10.178. ISSN: 0141-8130. [[2021 journal impact factor 8.025]. Q1
Reynoso-Cereceda GI, Córdova-Aguilar MS, Valdez-Cruz NA, Trujillo-Roldán MA*. Shaken flasks by resonant acoustic mixing in the biosynthesis of alginate by Azotobacter vinelandii with non-Newtonian rheological characteristics. Journal of Chemical Technology & Biotechnology (2018). 93(4):1159-1168. doi: 10.1002/jctb.5475. Online ISSN: 1097-4660. [2021 journal impact factor 3.709]. Q2

Circuito de Posgrado, Ciudad Universitaria
Alcaldía Coyoacán, C.P. 04510, México, CDMX

55 5623 7006
mdcbq@posgrado.unam.mx