Biología Molecular y Bioquímica de venenos de animales ponzoñosos.

Aislamiento y caracterización química y funcional de enzimas y de toxinas de venenos de reptiles.

Desarrollo y mejoramiento de antivenenos.

Farmacocinética de antivenenos en un modelos animal grande (borregos).

Publicaciones

141. Sanchez,M., Solano,G., Vargas,M., Reta-Mares,F., Neri-Castro,E., Alagon,A., Sanchez,A., Villalta,M., Leon,G., Segura,A. (2020). Toxicological profile of medically relevant Crotalus species from Mexico and their neutralization by a Crotalus basiliscus/Bothrops asper antivenom. Toxicon, 179, 92-100. doi.org/10.1016/j.toxicon.2020.03.006. (FI: 3.035).

142. Rivas-Mercado E., Neri-Castro E., Benard-Valle M., Rucavado-Romero,A., Olvera-Rodriguez A., Zamudio-Zuniga F., Alagon,A., Garza-Ocanas L. (2020). Disintegrins extracted from totonacan rattlesnake (Crotalus totonacus) venom and their anti-adhesive and anti-migration effects on MDA-MB-231 and HMEC-1 cells. Toxicology in Vitro, 65, 104809. doi.org/10.1016/j.tiv.2020.104809. (FI: 3.685).

143. Ponce-Lopez,R., Neri-Castro,E., Borja,M., Strickland,J.L., Alagon,A. (2020). Neutralizing potency and immunochemical evaluation of an anti-Crotalus mictlantecuhtli experimental serum. Toxicon, 187, 171-180. doi.org/10.1016/j.toxicon.2020.08.026. (FI: 3.035).

144. Paniagua, D., Crowns, K., Montonera, M., Wertheimer, A., Alagon, A, Boyer, L. Postmortem histopathology and detection of venom by ELISA following suicide by cobra (Naja kaouthia) envenomation. Forensic Toxicol. 38, 523–528 (2020). doi.org/10.1007/s11419-020-00535-w. (FI: 2.855).

145. Neri-Castro, E., Sanz, L., Olvera-Rodriguez, A., Benard-Valle, M., Alagon, A., Calvete,J.J. (2020). Venomics and biochemical analysis of the black-tailed horned pitviper, Mixcoatlus melanurus, and characterization of Melanurutoxin, a novel crotoxin homolog. Journal of Proteomics, 225, 103865. doi.org/10.1016/j.jprot.2020.103865. (FI: 3.855).

146. Neri-Castro,E., Benard-Valle,M., Paniagua,D., Boyer,L.V., Possani,D., Lopez-Casillas,F., Olvera,A., Romero,C., Zamudio,F., Alagon,A. (2020). Neotropical Rattlesnake (Crotalus simus) Venom Pharmacokinetics in Lymph and Blood Using an Ovine Model. Toxins (Basel), 12 (7), 455. doi.org/10.3390/toxins12070455. (FI:5.075).

147. Grashof,D., Zdenek,C.N., Dobson,J.S., Youngman,N.J., Coimbra,F., Benard-Valle,M., Alagon,A., Fry,B.G. (2020). A Web of Coagulotoxicity: Failure of Antivenom to Neutralize the Destructive (Non-Clotting) Fibrinogenolytic Activity of Loxosceles and Sicarius Spider Venoms. Toxins (Basel), 12 (2). doi.org/10.3390/toxins12020091. (FI:5.075).

148. Garcia-Osorio,B., Lomonte,B., Benard-Valle,M., Lopez de Leon J., Roman-Dominguez,L., Mejia-Dominguez,N., Lara-Hernandez,F., Alagon,A., Neri-Castro,E. (2020). Ontogenetic changes in the venom of Metlapilcoatlus nummifer, the Mexican jumping viper. Toxicon, 184, 204-214. doi.org/10.1016/j.toxicon.2020.06.023. (FI: 3.035).

149. Chippaux,J.P., Celis,A., Boyer,L., Alagon,A. (2020). Factors involved in the resilience of incidence and decrease of mortality from scorpion stings in Mexico. Toxicon, 188, 65-75. doi.org/10.1016/j.toxicon.2020.10.011. (FI: 3.035).

150. Braga, J.R.M., Jorge, A.R.C., Marinho, A.D., de Moraes Silveira, J.A., Nogueira-Junior, F.A., Benard-Valle, M., Alagon, A., de Menezes, R.R.P.P., Martins, A.M.C., Feijao, L.X., Monteiro, H.S.A., Jorge, R.J.B. (2020). Renal effects of venoms of Mexican coral snakes Micrurus browni and Micrurus laticollaris. Toxicon, 181, 45-52. doi.org/10.1016/j.toxicon.2020.04.095. (FI: 3.035).

151. Benard-Valle, M., Neri-Castro, E.E., Yanez-Mendoza, M.F., Lomonte, B., Olvera, A., Zamudio, F., Restano-Cassulini, R., Possani, L.D., Jimenez-Ferrer, E., Alagon, A. (2020). Functional, proteomic and transcriptomic characterization of the venom from Micrurus browni browni: Identification of the first lethal multimeric neurotoxin in coral snake venom. Journal of Proteomics, 225, 103863. doi.org/10.1016/j.jprot.2020.103863. (FI: 3.855).

152. Seneci, L., Zdenek, C.N., Bourke, L.A., Cochran, C., Sanchez, E.E., Neri-Castro, E., Benard-Valle, M., Alagon, A., Frank, N., Fry, B.G. (2021). A symphony of destruction: Dynamic differential fibrinogenolytic toxicity by rattlesnake (Crotalus and Sistrurus) venoms. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 245, 109034. doi.org/10.1016/j.cbpc.2021.109034. (FI: 3.228).

153. Seneci, L., Zdenek, C.N., Chowdhury, A., Rodrigues, C.F.B., Neri-Castro, E., Benard-Valle, M., Alagon, A., Fry, B.G. (2021). A Clot Twist: Extreme Variation in Coagulotoxicity Mechanisms in Mexican Neotropical Rattlesnake Venoms. Frontiers in Immunology, 12, 612846. doi.org/10.3389/fimmu.2021.612846. (FI: 6.429).

154. Rodrigues, C.F.B., Zdenek, C.N., Serino-Silva, C., de Morais-Zani, K., Grego, K.F., Benard-Valle, M., Neri-Castro, E., Alagon, A., Tanaka-Azevedo, A.M., Fry, B.G. (2021). Boa g PLI from Boa constrictor Blood is a Broad-Spectrum Inhibitor of Venom PLA2 Pathophysiological Actions. Journal of Chemical Ecology, 47, 907–914 (2021). doi.org/10.1007/s10886-021-01289-4. (FI: 2.239).

155. Ponce-Lopez, R., Neri-Castro, E., Olvera-Rodriguez, F., Sanchez, E.E., Alagon, A., Olvera-Rodriguez, A. (2021). Neutralization of crotamine by polyclonal antibodies generated against two whole rattlesnake venoms and a novel recombinant fusion protein. Toxicon, 197, 70-78. doi.org/10.1016/j.toxicon.2021.04.005. (FI: 3.035).

156. Franco-Servin, C., Neri-Castro, E., Benard-Valle, M., Alagon, A., Rosales-Garcia, R.A., Guerrero-Alba, R., Poblano-Sanchez, J.E., Silva-Briano, M., Guerrero-Barrera, A.L., Sigala-Rodriguez, J.J. (2021). Biological and Biochemical Characterization of Coronado Island Rattlesnake (Crotalus helleri caliginis) Venom and Antivenom Neutralization. Toxins (Basel), 13 (8), 582. doi.org/10.3390/toxins13080582. (FI:5.075).

157. de Roodt, A.R., Lanari, L.C., Ramirez, J.E., Gomez, C., Barragan, J., Litwin, S., van Grootheest, J.H., Desio, M., Dokmetjian, J.C., Dolab, J.A., Damin, C.F., Alagon, A. (2021). Cross-reactivity of some Micrurus venoms against experimental and therapeutic anti-micrurus antivenoms. Toxicon, 200, 153-164. doi.org/10.1016/j.toxicon.2021.07.011. (FI: 3.035).

158. Dashevsky, D., Benard-Valle, M., Neri-Castro, E., Youngman, N.J., Zdenek, C.N., Alagon, A., Portes-Junior, J.A., Frank, N., Fry, B.G. (2021). Anticoagulant Micrurus venoms: targets and neutralization. Toxicology Letters, 337, 91-97. doi.org/10.1016/j.toxlet.2020.11.010. (4.372).

159. Colis-Torres, A., Neri-Castro, E., Strickland, J.L., Olvera-Rodriguez, A., Borja, M., Calvete, J., Jones, J., Parkinson, C.L., Banuelos, J., Lopez de Leon, J., Alagon, A. (2021). Intraspecific venom variation of Mexican West Coast Rattlesnakes (Crotalus basiliscus) and its implications for antivenom production. Biochimie, 192: 111-124. doi.org/10.1016/j.biochi.2021.10.006. (FI: 4.372).

160. Bourke, L.A., Zdenek, C.N., Neri-Castro, E., Benard-Valle, M., Alagon, A., Gutierrez, J.M., Sanchez, E. F., Aldridge, M., Fry, B.G. (2021). Pan-American Lancehead Pit-Vipers: Coagulotoxic Venom Effects and Antivenom Neutralisation of Bothrops asper and B. atrox Geographical Variants. Toxins (Basel), 13 (2), 78. doi.org/10.3390/toxins13020078. (FI: 5.075)

161. Benard-Valle, M., Neri-Castro, E., Elizalde-Morales, N., Olvera-Rodriguez, A., Strickland, J., Acosta, G., Alagon, A. (2021). Protein composition and biochemical characterization of venom from Sonoran Coral Snakes (Micruroides euryxanthus). Biochimie, 182, 206-216. doi.org/10.1016/j.biochi.2021.01.003. (FI: 4.372).

162. Archundia, I.G., de la Rosa, G., Olvera, F., Calderon, A., Benard-Valle, M., Alagon, A., Corzo, G. (2021). Assessment of neutralization of Micrurus venoms with a blend of anti-Micrurus tener and anti-ScNtx antibodies. Vaccine, 39 (6), 1000-1006. doi.org/10.1016/j.vaccine.2020.12.052. (FI:4169).

163. Youngman, N.J., Carlsson, D.J., Jones, L., Neri-Castro, E., Alagon, A., Fry, B.G. (2022). Cloud serpent coagulotoxicity: The biochemical mechanisms underpinning the anticoagulant actions of Mixcoatlus and Ophryacus venoms. Toxicon, 211, 44-49. doi: 10.1016/j.toxicon.2022.03.009 . (FI: 3.035).

164. Neri-Castro, E., Strickland, J.L., Carbajal-Marquez, R.A., Zuniga, J., Ponce-Lopez, R., Olvera, F., Alagon, A. (2022). Characterization of the venom and external morphology of a natural hybrid between Crotalus atrox and Crotalus mictlantecuhtli. Toxicon, 207, 43-47. doi: 10.1016/j.toxicon.2022.01.003 . (FI: 3.035).

165. Colis-Torres, A., Neri-Castro, E., Strickland, J.L., Olvera-Rodriguez, A., Borja, M., Calvete, J., Jones, J., Parkinson, C.L., Banuelos, J., Lopez de Leon, J., Alagon, A. (2022). Intraspecific venom variation of Mexican West Coast Rattlesnakes (Crotalus basiliscus) and its implications for antivenom production. Biochimie, 192, 114-124. doi: 10.1016/j.biochi.2021.10.006. (FI: 4.372).

166. Olvera Rodríguez, A., Amaro Ruiz, MG., Bénard-Valle, M., Neri-Castro, E., Olvera
Rodríguez, F., Alagón, A. (2022) Neutralization of black widow spider (Latrodectus mactans) venom with rabbit polyclonal serum hyperimmunized with recombinant alpha-latrotoxin fragments. Biochimie. 201:55-62. doi:10.1016/j.biochi.2022.06.012. (FI: 4.372).

167. Neri-Castro, E., Zarzosa, V., Colis-Torres, A., Fry, BG., Olvera-Rodríguez, A., Jones, J., Reyes-Velasco, J., Zamudio, F., Borja, M., Alagón, A., Lomonte, B. Proteomic and toxicological characterization of the venoms of the most enigmatic group of rattlesnakes: The long-tailed rattlesnakes. (2022) Biochimie, 202, 226-236. doi: 10.1016/j.biochi.2022.08.015. (FI: 4.372).

168. Siegrist, A.A., Boyer, L., Balchan, N.R., Vázquez, H., Alagón, A., de Roodt, A., Craig, I.S., Poulin, I.S., Timmerman, A., Trocola, D. (2023). Snake and Scorpion Antivenom in Birds of Prey. Journal of Zoo and Wildlife Medicine, 53(4), 870-806, doi: 10.1638/2021-0124. (FI: 0.806).

169. Borja, M., Neri-Castro, E., Gutiérrez Martínez, A., Bledsoe, R., Zarzosa, V., Rodriguez-Lopez, B., Strickland, J.L., Becerra-López, J., Valenzuela-Ceballos, S., Parkinson, C.L., Alagón, A., Castañeda-Gaytán, G. (2023). Ontogenetic change in the venom composition of one Mexican black-tailed rattlesnake (Crotalus molossus nigrescens) from Durango, Mexico. Toxicon, 24, 107280. https://doi.org/10.1016/j.toxicon.2023.107280

170. Perez-Juarez,H., Serrano-Vazquez,A., Godinez-Alvarez,H., Gonzalez,E., Rojas-Velazquez,L., Moran,P., Portillo-Bobadilla,T., Ramiro,M., Hernandez,E., Lau,C., Martinez,M., Padilla,M.L.A., Zaragoza,M.E., Taboada,B., Palomares,L.A., Lopez,S., Alagon,A., Arias,C.F., Ximenez,C. (2023). Longitudinal anti-SARS-CoV-2 antibody immune response in acute and convalescent patients. Frontiers in Cellular and Infection Microbiology, 13, 1239700. https://doi.org/10.3389/fcimb.2023.1239700

171. Benard-Valle,M., Wouters,Y., Ljungars,A., Nguyen,G.T.T., Ahmadi,S., Ebersole,T.W., Dahl,C.H., Guadarrama-Martinez,A., Jeppesen,F., Eriksen,H., Rodriguez-Barrera,G., Boddum,K., Jenkins,T.P., Bjorn,S.P., Schoffelen,S., Voldborg,B.G., Alagon,A., Laustsen,A.H. (2024). In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model. Nature Communications, 15 (1), 4310. https://doi.org/10.1038/s41467-024-48539-z
172. Guadarrama-Martinez,A., Neri-Castro,E., Boyer,L., Alagon,A. (2024). Variability in antivenom neutralization of Mexican viperid snake venoms. PLoS Neglected Tropical Diseases, 18 (5), e0012152. https://doi.org/10.1371/journal.pntd.0012152

173. Bourke,L.A., Zdenek,C.N., Huynh,T.M., Hodgson,W.C., Alagon,A., Castro,E.N., Jones,J., Fry,B.G. (2024). Fangs and Foliage: Unearthing the Haemotoxic Secrets of Cannabis-Dwelling Rattlesnakes. Toxicon, 244, 107756. https://doi.org/10.1016/j.toxicon.2024.107756

174. Dashevsky,D., Harris,R.J., Zdenek,C.N., Benard-Valle,M., Alagon,A., Portes-Junior,J.A., Tanaka-Azevedo,A.M., Grego,K.F., Sant'Anna,S.S., Frank,N., Fry,B.G. (2024). Red-on-Yellow Queen: Bio-Layer Interferometry Reveals Functional Diversity Within Micrurus Venoms and Toxin Resistance in Prey Species. Journal of Molecular Evolution, 92, 317-328. https://doi.org/10.1007/s00239-024-10176-x

175. Neri-Castro,E., Zarzosa,V., Benard-Valle,M., Rodriguez-Solis,A.M., Hernandez-Orihuela,L., Ortiz-Medina,J.A., Alagon,A. (2024). Quantifying venom production: A study on Micrurus snakes in Mexico. Toxicon, 240, 107658. https://doi.org/10.1016/j.toxicon.2024.107658

176. Neri-Castro,E., Zarzosa,V., Lomonte,B., Zamudio,F., Hernandez-Orihuela,L., Olvera-Rodriguez,A., Rodriguez-Solis,A.M., Borja,M., Garcia-Vazquez,U.O., Jones,J.M., Parkinson,C.L., Alagon,A. (2024). Exploring Venom Diversity in Mixcoatlus browni and Mixcoatlus barbouri: A Comparative Analysis of Two Rare Mexican Snake Species with Crotoxin-like Presence. Biochimie, 225, 81-88.

177, Olivares-Hernandez,R., Riano-Umbarila,L., Becerril,B., Alagon-Cano,A., Vazquez-Lopez,H. (2024). Pharmacokinetic evaluation of a single chain antibody fragment against scorpion toxins in sheep. Toxicon, 242, 107691.

178. Valdez-Cruz,N.A., Rosiles-Becerril,D., Martinez-Olivares,C.E., Garcia-Hernandez,E., Cobos-Marin,L., Garzon,D., Lopez-Salas,F.E., Zavala,G., Luviano,A., Olvera,A., Alagon,A., Ramirez,O.T., Trujillo-Roldan,M.A (2024). Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19. Microbial Cell Factories, 23 (1), 41.

179. Zarzosa,V., Lomonte,B., Zamudio,F., Ponce-Lopez,R., Olvera-Rodriguez,F., Borja,M., Alagon,A., Neri-Castro,E. (2024). Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits. Biochimie, 216, 160-174.

180. Campbell,S.I.D., Chow,C.Y., Neri-Castro,E., Alagon,A., Gomez,A., Soria,R., King,G.F., Fry,B.G. (2024). Taking the sting out of scorpions: Electrophysiological investigation of the relative efficacy of three antivenoms against medically significant Centruroides species. Comparative Biochemistry and Physiology C Toxicology and Pharmacology, 283, 109977.

182. Benard-Valle,M., Wouters,Y., Ljungars,A., Nguyen,G.T.T., Ahmadi,S., Ebersole,T.W., Dahl,C.H., Guadarrama-Martinez,A., Jeppesen,F., Eriksen,H., Rodriguez-Barrera,G., Boddum,K., Jenkins,T.P., Bjorn,S.P., Schoffelen,S., Voldborg,B.G., Alagon,A., Laustsen,A.H. (2024). In vivo neutralization of coral snake venoms with an oligoclonal nanobody mixture in a murine challenge model. Nature Communications, 15 (1), 4310

Circuito de Posgrado, Ciudad Universitaria
Alcaldía Coyoacán, C.P. 04510, México, CDMX

55 5623 7006
mdcbq@posgrado.unam.mx