Contacto
Campos de conocimiento
Biofísica
Fisiología Molecular
Genética y Fisiología Celular
Líneas de investigación
Fisiología Celular y Bioquímica. Canales iónicos. Fisiología del espermatozoide.
Publicaciones
2019
• Brukman NG, Nuñez SY, Puga Molina LDC, Buffone MG, Darszon A, Cuasnicu PS, Da Ros VG. Tyrosine phosphorylation signaling regulates Ca(2+) entry by affecting intracellular pH during human sperm capacitation. J Cell Physiol. 2019. doi: 10.1242/jcs. PMID: 303031778.
• Zhao R, Kennedy K, De Blas GA, Orta G, Pavarotti MA, Arias RJ, de la Vega-Beltrán JL, Li Q, Dai H, Perozo E, Mayorga LS, Darszon A., Goldstein SAN. Role of human Hv1 channels in sperm capacitation and white blood cell respiratory burst established by a designed peptide inhibitor. Proc Natl Acad Sci U S A. 2018 Dec 11;115: E11847- E11856. doi:10.1073/pnas.1816189115. PubMed PMID: 30478045.
• . Bondarenko O, Corzo G, Santana FL, Del Río-Portilla F, Darszon A, López-González I. Nonenzymatically oxidized arachidonic acid regulates T-type Ca(2+) currents in mouse spermatogenic cells. FEBS Lett. 2019 593: 1735-1750. doi:10.1002/1873-3468.13448. PubMed PMID: 31115913.
• Ramírez-Gómez HV, Tuval I, Guerrero A, Darszon A. Analysis of sperm chemotaxis. Methods Cell Biol.2019;151:473-486. doi: 10.1016/bs.mcb.2018.12.002. Review. PubMed PMID: 30948027.
• Bastián-Eugenio CE, Bohórquez-Hernández A, Pacheco J, Sampieri A, Asanov A, Ocelotl-Oviedo JP, Guerrero A, Darszon A, Vaca L. Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels modulates cell migration and wound healing. Commun Biol. 2019. 2: 88. doi: 10.1038/s42003-019-0338-1.
• Navarrete FA, Aguila L, Martin-Hidalgo D, Tourzani DA, Luque GM, Ardestani G, Garcia Vazquez FA, Levin LR, Buck J, Darszon A, Buffone MG, Mager J, Fissore RA, Salicioni AM, Gervasi MG, Visconti PE. Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies. Front Cell Dev Biol. 2019. 7:262.
• Bondarenko O, Corzo G, Santana FL, Del Río-Portilla F, Darszon A, López-González I. Nonenzymatically oxidized arachidonic acid regulates T-type Ca 2+ currents in mouse spermatogenic cells. FEBS Lett. 2019. 593:1735-1750. doi: 10.1002/1873-3468.13448. Epub 2019 Jun 6. PMID: 31115913.
2020
• Darszon A, Nishigaki T, López-González I, Visconti PE, Treviño CL. Differences and Similarities: The Richness of Comparative Sperm Physiology. Physiology (Bethesda). 2020 May 1;35(3):196-208. doi: 10.1152/physiol.00033.2019.
• PMID: 32293232.
• Ramírez-Gómez HV, Jimenez Sabinina V, Velázquez Pérez M, Beltran C, Carneiro J, Wood CD, Tuval I, Darszon A, Guerrero A. Sperm chemotaxis is driven by the slope of the chemoattractant concentration field. Elife. 2020 Mar9;9:e50532. doi: 10.7554/eLife.50532. PMID: 32149603; PMCID: PMC7093112.
• Priego-Espinosa DA, Darszon A, Guerrero A, González-Cota AL, Nishigaki T, Martínez-Mekler G, Carneiro J.Modular analysis of the control of flagelar Ca 2+ -spike trains produced by CatSper and CaV channels in sea urchin sperm. PLoS Comput Biol. 2020. 16:e1007605. doi: 10.1371/journal.pcbi.1007605. PMID: 32119665; PMCID: PMC7067495.
• Chávez JC, Darszon A, Treviño CL, Nishigaki T. Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F. Front Cell Dev Biol. 2020 Jan 17;7:366. doi: 10.3389/fcell.2019.00366. PMID: 32010689; PMCID: PMC6978660.
• Garza-López E, Aldana A, Darszon A, Nishigaki T, López-González I. Ca V 3.1 channel pore pseudo- symmetry revealed by selectivity filter mutations in its domains I/II. Cell Calcium. 2020. 89:102214. doi: 10.1016/j.ceca.2020.102214. Epub 2020 May 11. PMID: 32428730.
• Balestrini PA, Jabloñski M, Schiavi-Ehrenhaus LJ, Marín-Briggiler CI, Sánchez-Cárdenas C, Darszon A, Krapf D,Buffone MG. Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time. Mol Reprod Dev. 2020 Dec;87(12):1188-1198. doi: 10.1002/mrd.23431.
2021
• Corkidi G, Hernández-Herrera P, Montoya F, Gadêlha H, Darszon A. Long-term segmentation-free assessment of head-flagellum movement and intracellular calcium in swimming human sperm. J Cell Sci. 2021;134(3):jcs250654. doi: 10.1242/jcs.250654. PMID: 33431515.
• Mata-Martínez E, Sánchez-Tusie AA, Darszon A, Mayorga LS, Treviño CL, De Blas, GA. Epac activation induces an extracellular Ca 2+ -independent Ca 2+ wave that triggers acrosome reaction in human spermatozoa. Andrology. 2021 Feb 20. doi: 10.1111/andr.12989. Epub ahead of print. PMID: 33609309.
• Sánchez-Cárdenas C, Romarowski A, Orta G, De la Vega-Beltrán JL, Martín-Hidalgo D, Hernández-Cruz A, Visconti PE, Darszon A. Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction. FASEB J. 2021 Apr;35(4):e21528. doi:10.1096/fj.202100122R. PMID: 33742713. 20
• Zhao R, Dai H, Arias RJ, De Blas GA, Orta G, Pavarotti MA, Shen R, Perozo E, Mayorga LS, Darszon A,Goldstein SAN. Direct activation of the proton channel by albumin leads to human sperm capacitation and sustained
• release of inflammatory mediators by neutrophils. Nat Commun. 2021 Jun 22;12(1):3855. doi:
• 10.1038/s41467-021-24145-1. PMID: 34158477; PMCID: PMC8219737.
• Luque GM, Xu X, Romarowski A, Gervasi MG, Orta G, De la Vega-Beltrán JL, Stival C, Gilio N, Dalotto-Moreno T, Krapf D, Visconti PE, Krapf D, Darszon A, Buffone MG. Cdc42 localized in the CatSper signaling complex regulates cAMP-dependent pathways in mouse sperm. FASEB J. 2021 Aug;35(8):e21723. doi: 10.1096/fj.202002773RR. PMID: 34224609.
• Balestrini PA, Sanchez-Cardenas C, Luque GM, Baro Graf C, Sierra JM, Hernández-Cruz A, Visconti PE, Krapf D,Darszon A, Buffone MG. Membrane hyperpolarization abolishes calcium oscillations that prevent induced acrosomal exocytosis in human sperm. FASEB J. 2021 Jun;35(6):e21478. doi: 10.1096/fj.202002333RR. PMID: 33991146.
• Aldana A, Carneiro J, Martínez-Mekler G, Darszon A. Discrete Dynamic Model of the Mammalian Sperm Acrosome Reaction: The Influence of Acrosomal pH and Physiological Heterogeneity. Frontiers in Physiology. 2021. 12: 932. doi:10.3389/fphys.2021.682790.
• Aguado-García A, Priego-Espinosa DA, Aldana A, Darszon A, Martínez-Mekler G. Mathematical model reveals
• that heterogeneity in the number of ion transporters regulates the fraction of mouse sperm capacitation. PLoS One. 2021Nov 18;16(11):e0245816. doi: 10.1371/journal.pone.0245816. PMID: 34793454; PMCID: PMC8601445.
• Loyo-Celis V, Orta G, Beltrán C, Darszon A. CatSper channels in sea urchin sperm. Cell Calcium. 2021Nov;99:102466. doi: 10.1016/j.ceca.2021.102466. Epub 2021 Aug 31. PMID: 34509139.
• Mata-Martínez E, Sánchez-Cárdenas C, Chávez JC, Guerrero A, Treviño CL, Corkidi G, Montoya F, Hernandez- Herrera P, Buffone MG, Balestrini PA, Darszon A. Role of calcium oscillations in sperm physiology. in: Biosystems. 2021 Dec;210:104546. PMID: 34453988.
• Aldana A, Carneiro J, Martínez-Mekler G, Darszon A. Discrete Dynamic Model of the Mammalian Sperm Acrosome Reaction: The Influence of Acrosomal pH and Physiological Heterogeneity. Front Physiol. 2021 Jul 19;12:682790. doi:10.3389/fphys.2021.682790. PMID: 34349664; PMCID: PMC8328089.
2022
• Yang, F., Gervasi, M.G., Leu, N.A., Orta, G., Tourzani, D.A., De la Vega-Beltran, J.L., Ruthel, G.,
• Darszon, A., Visconti, P.E. and Wang, J. C2CD6 regulates targeting and organization of the CatSper calcium channel complex in sperm flagella. Development. 2022. 149(2): dev199988. doi: 10.1242/dev.199988. Epub Jan 14.PMID: 34919125.
• Loza-Huerta A, Pacheco-Castillo H, Darszon A, Beltrán C. Crosstalk between protein kinases A and Cregulates sea urchin sperm motility. Zygote. 2022. 30(3):398-409. doi: 10.1017/S0967199421000915. Epub 2021 Dec 2.PMID: 34852867232.
• Carrasquel Martínez G, Aldana A, Carneiro J, Treviño CL, Darszon A. Acrosomal alkalinization occurs during human sperm capacitation. Mol Hum Reprod. 2022 Feb 24: gaac005. doi: 10.1093/molehr/gaac005. Epub ahead of print. PMID: 35201340.
• Hernandez HO, Hernandez-Herrera P, Montoya F, Olveres J, Bloomfield-Gadelh H, Darszon A,
• Escalante-Ramirez B, Corkidi G. 3D+t feature-based descriptor for unsupervised flagellar human sperm beat classification. Annu Int Conf IEEE Eng Med Biol Soc. 2022 Jul;2022:488-492. Doi 10.1109/EMBC48229.2022.9871419. PMID: 36085948.
• Torres-García E, Pinto-Cámara R, Linares A, Martínez D, Abonza V, Brito-Alarcón E, Calcines-Cruz C, Valdés-Galindo G, Torres D, Jabloñski M, Torres-Martínez HH, Martínez JL, Hernández HO, Ocelotl-OviedoJP, Garcés Y, Barchi M, D'Antuono R, Bošković A, Dubrovsky JG, Darszon A, Buffone MG, Morales RR, Rendon-Mancha JM, Wood CD, Hernández-García A, Krapf D, Crevenna ÁH, Guerrero A. Extending resolution within a single imaging frame. Nat Commun. 2022 Dec 2;13(1):7452. doi: 10.1038/s41467-022- 34693-9. PMID: 36460648; PMCID: PMC9718789.
2023
• López-González I, Sánchez-Cárdenas C, De la Vega-Beltrán JL, Alvarado-Quevedo B, Ocelotl-Oviedo JP, González-Cota AL, Aldana A, Orta G, Darszon A. ATP increases head volume in capacitated human sperm via a purinergic channel. Biochem Biophys Res Commun. 2023 Sep 3;671:318-326. doi: 10.1016/j.bbrc.2023.06.008. Epub 2023 Jun 3. PMID: 37327703.
• Oliver EI, Jabloñski M, Buffone MG, Darszon A. Two-pore channel 1 and Ca<sup>2+</sup> release- activated Ca<sup>2+</sup> channels contribute to the acrosomal pH-dependent intracellular Ca<sup>2+</sup> increase in mouse sperm. J Physiol. 2023 Jul;601(14):2935-2958. doi: 10.1113/JP284247. Epub 2023 Jun 6. PMID: 37278367.
• Luque GM, Schiavi-Ehrenhaus LJ, Jabloñski M, Balestrini PA, Novero AG, Torres NI, Osycka-Salut CE, Darszon A, Krapf D, Buffone MG. High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding. Front Cell Dev Biol. 2023 Jan 19;11:1010306. doi: 10.3389/fcell.2023.1010306. PMID: 36743410; PMCID: PMC9892719.21
• Corkidi G, Montoya F, González-Cota AL, Hernández-Herrera P, Bruce NC, Bloomfield-Gadêlha H,Darszon A. Human sperm rotate with a conserved direction during free swimming in four dimensions. J CellSci. 2023 Nov 15;136(22):jcs261306. doi: 10.1242/jcs.261306. Epub 2023 Nov 29. PMID: 37902031;PMCID: PMC10729817.
2024
• Chávez JC, Carrasquel-Martínez G, Hernández-Garduño S, Matamoros Volante A, Treviño CL,
• Nishigaki T, Darszon A. Cytosolic and Acrosomal pH Regulation in Mammalian Sperm. Cells. 2024 May 17;13(10):865. doi: 10.3390/cells13100865. PMID: 38786087; PMCID: PMC11120249.
• Hernández HO, Montoya F, Hernández-Herrera P, Díaz-Guerrero DS, Olveres J, Bloomfield-Gadêlha H,Darszon A, Escalante-Ramírez B, Corkidi G. Feature-based 3D+t descriptors of hyperactivated human spermbeat patterns. Heliyon. 2024 Feb 23;10(5):e26645. doi: 10.1016/j.heliyon.2024.e26645. PMID: 38444471;PMCID: PMC10912238.
• López-González I, Oseguera-López I, Castillo R, Darszon A. Influence of extracellular ATP on
• mammalian sperm physiology. Reprod Fertil Dev. 2024 Jun;36:RD23227. doi: 10.1071/RD23227. PMID:38870344.
• Priego Espinosa D, Espinal-Enríquez J, Aldana A, Aldana M, Martínez-Mekler G, Carneiro J, DarszonA. Reviewing mathematical models of sperm signaling networks. Mol Reprod Dev. 2024 Aug;91(8):e23766.doi: 10.1002/mrd.23766. PMID: 39175359.
• Novero AG, Rodríguez PT, De la Vega Beltrán JL, Schiavi-Ehrenhaus LJ, LuqueGM, Carruba M, Stival C, Gentile I, Ritagliati C, Santi CM, Nishigaki T, Krapf D, Buffone MG, Darszon A, Treviño CL, Krapf D.The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse
• sperm. J Biol Chem. 2024 Oct 28:107932. doi: 10.1016/j.jbc.2024.107932. Epub ahead of print. PMID:39476963.
• Jabloñski M, Luque GM, Gómez-Elías MD, Sanchez-Cardenas C, Xu X, de la Vega-Beltran JL, CorkidiG, Linares A, Abonza Amaro VX, Arenas-Hernandez A, Del Pilar Ramos-Godinez M, López-Saavedra A,Krapf D, Krapf D, Darszon A, Guerrero A, Buffone MG. Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization. Elife 2024 Nov 13;13:RP93792. doi: 10.7554/eLife.93792. PMID: 39535529; PMCID: PMC11560130.
• Sánchez-Cárdenas C, De la Vega-Beltrán JL, Weber WD, Orta G, Sánchez-Guevara Y, Hernández-Cruz A, Darszon A, Visconti PE. Intracellular Mg<sup>2+</sup> concentrations are differentially regulated in the sperm head and mid-piece in acrosome reaction inducing conditions. FASEB J. 2024 Nov 15;38(21):e70129. doi: 10.1096/fj.202401243R. PMID: 39530594.
