Contacto
Campos de conocimiento
Bioquímica
Biotecnología
Microbiología Molecular
Líneas de investigación
Analisis de oligomerización de las toxinas Cry producidas por la bacteria Bacillus thuringiensis.
Edición genetica de insectos con CRISPR-Cas.
Evolucion dirigida de toxinas Cry.
Publicaciones
1. Pacheco, S., Gómez, I., Chiñas, M., Sánchez, J., Soberón, M. and Bravo, A. (2021). Whole Genome Sequencing Analysis of Bacillus thuringiensis GR007 Reveals Multiple Pesticidal Protein Genes. Front. Microbiol. 12:758314.
2. López-Molina, S., do Nascimento, N. A., Silva-Filha, M., Guerrero, A., Sánchez, J., Pacheco, S., Gill, S. S., Soberón, M., & Bravo, A. (2021). In vivo nanoscale analysis of the dynamic synergistic interaction of Bacillus thuringiensis Cry11Aa and Cyt1Aa toxins in Aedes aegypti. PLoS pathogens, 17(1), e1009199.
3. Sena da Silva, I. H., Gómez, I., Pacheco, S., Sánchez, J., Zhang, J., Luque Castellane, T. C., Aparecida Desiderio, J., Soberón, M., Bravo, A., & Polanczyk, R. A. (2021). Bacillus thuringiensis Cry1Ab Domain III β-16 Is Involved in Binding to Prohibitin, Which Correlates with Toxicity against Helicoverpa armigera (Lepidoptera: Noctuidae). Applied and environmental microbiology, 87(2), e01930-20.
4. Pacheco, S., Quiliche, J., Gómez, I., Sánchez, J., Soberón, M., & Bravo, A. (2020). Rearrangement of N-Terminal α-Helices of Bacillus thuringiensis Cry1Ab Toxin Essential for Oligomer Assembly and Toxicity. Toxins, 12(10), 647.
5. Onofre, J., Pacheco, S., Torres-Quintero, M. C., Gill, S. S., Soberon, M., and Bravo, A. (2020) The Cyt1Aa toxin from Bacillus thuringiensis inserts into target membranes via different mechanisms in insects, red blood cells, and lipid liposomes, The Journal of biological chemistry 295, 9606-9617.
6. Chimal-Cázares, F., Hernández-Martínez, G., Pacheco, S., Ares, M. A., Soria-Bustos, J., Sánchez-Gutiérrez, M., Izquierdo-Vega, J. A., Ibarra, J. A., González-y-Merchand, J. A., Gorvel, J.-P., Méresse, S., and De la Cruz, M. A. (2020) Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence, Frontiers in Microbiology 11.
7. Nascimento, N. A., Torres-Quintero, M. C., Molina, S. L., Pacheco, S., Romao, T. P., Pereira-Neves, A., Soberon, M., Bravo, A., and Silva-Filha, M. (2020) Functional Bacillus thuringiensis Cyt1Aa Is Necessary To Synergize Lysinibacillus sphaericus Binary Toxin (Bin) against Bin-Resistant and -Refractory Mosquito Species, Appl Environ Microbiol 86.
8. Wang, Z., Fang, L., Zhou, Z., Pacheco, S., Gomez, I., Song, F., Soberon, M., Zhang, J., and Bravo, A. (2018) Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis), J Biol Chem 293, 11447-11458.
9. Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B. I., Czajkowsky, D. M., Zhang, J., Soberon, M., and Bravo, A. (2018) Helix alpha-3 inter-molecular salt bridges and conformational changes are essential for toxicity of Bacillus thuringiensis 3D-Cry toxin family, Sci Rep 8, 10331.
10. Gomez, I., Rodriguez-Chamorro, D. E., Flores-Ramirez, G., Grande, R., Zuniga, F., Portugal, F. J., Sanchez, J., Pacheco, S., Bravo, A., and Soberon, M. (2018) Spodoptera frugiperda (J. E. Smith) aminopeptidase N1 is functional receptor of Bacillus thuringiensis Cry1Ca toxin, Appl Environ Microbiol. 17;84(17).
11. Torres-Quintero, M. C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., and Bravo, A. (2018) Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity, Sci Rep 8, 4989.
12. Soberon, M., Portugal, L., Garcia-Gomez, B. I., Sanchez, J., Onofre, J., Gomez, I., Pacheco, S., and Bravo, A. (2018) Cell lines as models for the study of Cry toxins from Bacillus thuringiensis, Insect Biochem Mol Biol 93, 66-78.
13. Xiao, Y., Dai, Q., Hu, R., Pacheco, S., Yang, Y., Liang, G., Soberon, M., Bravo, A., Liu, K., and Wu, K. (2017) A Single Point Mutation Resulting in Cadherin Mislocalization Underpins Resistance against Bacillus thuringiensis Toxin in Cotton Bollworm, J Biol Chem 292, 2933-2943.
14. Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B. I., Soberon, M., and Bravo, A. (2017) An Intramolecular Salt Bridge in Bacillus thuringiensis Cry4Ba Toxin Is Involved in the Stability of Helix alpha-3, Which Is Needed for Oligomerization and Insecticidal Activity, Appl Environ Microbiol 83.
15. Onofre, J., Gaytan, M. O., Pena-Cardena, A., Garcia-Gomez, B. I., Pacheco, S., Gomez, I., Bravo, A., and Soberon, M. (2017) Identification of Aminopeptidase-N2 as a Cry2Ab binding protein in Manduca sexta, Peptides 98, 93-98.
16. De la Cruz, M. A., Ruiz-Tagle, A., Ares, M. A., Pacheco, S., Yanez, J. A., Cedillo, L., Torres, J., and Giron, J. A. (2017) The expression of Longus type 4 pilus of enterotoxigenic Escherichia coli is regulated by LngR and LngS and by H-NS, CpxR and CRP global regulators, Environ Microbiol 19, 1761-1775.
17. Ares, M. A., Rios-Sarabia, N., De la Cruz, M. A., Rivera-Gutierrez, S., Garcia-Morales, L., Leon-Solis, L., Espitia, C., Pacheco, S., Cerna-Cortes, J. F., Helguera-Repetto, C. A., Garcia, M. J., and Gonzalez, Y. M. J. A. (2017) The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments, New Microbiol 40, 199-204.
18. Ares, M. A., Fernandez-Vazquez, J. L., Pacheco, S., Martinez-Santos, V. I., Jarillo-Quijada, M. D., Torres, J., Alcantar-Curiel, M. D., Gonzalez, Y. M. J. A., and De la Cruz, M. A. (2017) Additional regulatory activities of MrkH for the transcriptional expression of the Klebsiella pneumoniae mrk genes: Antagonist of H-NS and repressor, PLoS One 12, e0173285.
