-Optimización de alfa-amilasas para la síntesis de alquilglucósidos. Ingeniería de enzimas mediante evolución dirigida y diseño racional.

-Mecanismos de especificidad de las enzimas desubiquitinasas. Ubiquitinación, reconocimiento específico de cadenas de poliubiquitina y actividad hidrolítica.

Publicaciones

1. Miranda-Molina A, Xolalpa W, Strompen S, Arreola-Barroso R, Olvera L, López-Munguía A, Castillo E, Saab-Rincon G. (2019) Deep Eutectic Solvents as New Reaction Media to Produce Alkyl-Glycosides Using Alpha-Amylase from Thermotoga maritima. Int J Mol Sci. 20(21). pii: E5439. doi: 10.3390/ijms20215439.
Journal IF 4.5

2. Lang V, Aillet F, Xolalpa W, Serna S, Ceccato L, Lopez-Reyes RG, Lopez-Mato MP, Januchowski R, Reichardt NC, Rodriguez MS. (2017) Analysis of defective protein ubiquitylation associated to adriamycin resistant cells. Cell Cycle. 16(24):2337-2344. doi: 10.1080/15384101.2017.1387694.
Journal IF 3.6

3. Velasco-Velázquez MA, Salinas-Jazmín N, Hisaki-Itaya E, Cobos-Puc L, Xolalpa W, González G, Tenorio-
Calvo A, Piña-Lara N, Juárez-Bayardo LC, Flores-Ortiz LF, Medina-Rivero E, Pérez NO, Pérez-Tapia SM.
(2017) Extensive preclinical evaluation of an infliximab biosimilar candidate. Eur J Pharm Sci. doi:
10.1016/j.ejps.2017.01.038.
Journal IF 3.6

4. Pirone L, Xolalpa W, Sigurðsson JO, Ramirez J, Pérez C, González M, de Sabando AR, Elortza F,
Rodriguez MS, Mayor U, Olsen JV, Barrio R, Sutherland JD. (2017) A comprehensive platform for the
analysis of ubiquitin-like protein modifications using in vivo biotinylation. Sci Rep. Jan 18;7:40756. doi:
10.1038/srep40756.
Journal IF 3.9

5. Pirone L, Xolalpa W, Mayor U, Barrio R, Sutherland JD. (2016) Analysis of SUMOylated Proteins in Cells
and In Vivo Using the bioSUMO Strategy. Methods Mol Biol. 2016;1475:161-9. doi: 10.1007/978-1-4939-
6358-4_12.

6. Xolalpa W, Rodriguez MS, England P. (2016) Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions Between SUMO Traps and Mono- or PolySUMO Moieties. Methods Mol Biol. 1475:99-107. doi: 10.1007/978-1-4939-6358-4_7.

7. Xolalpa W, Mata-Cantero L, Aillet F, Rodriguez MS. (2016) Isolation of the Ubiquitin-Proteome from Tumor Cell Lines and Primary Cells Using TUBEs. Methods Mol Biol. 1449:161-75. doi: 10.1007/978-1-4939-3756-1_8.

8. Serna S, Xolalpa W, Lang V, Aillet F, England P, Reichardt N, Rodriguez MS. (2016) Efficient monitoring of protein ubiquitylation levels using TUBEs-based microarrays. FEBS Lett. Aug;590(16):2748-56. doi: 10.1002/1873-3468.12289.
Journal IF 3.0

9. Mata-Cantero L, Azkargorta M, Aillet F, Xolalpa W, LaFuente MJ, Elortza F, Carvalho AS, Martin-Plaza J, Matthiesen R, Rodriguez MS. (2016) New insights into host-parasite ubiquitin proteome dynamics in P. falciparum infected red blood cells using a TUBEs-MS approach. J Proteomics. Apr 29;139:45-59. doi: 10.1016/j.jprot.2016.03.004.
Journal IF 3.5

Circuito de Posgrado, Ciudad Universitaria
Alcaldía Coyoacán, C.P. 04510, México, CDMX

55 5623 7006
mdcbq@posgrado.unam.mx